4

4-Title: Molecular detection of bovine rotavirus from north Gujarat, India

Authors: AI Dadawala, BS Chandel, HC Chauhan and AG Bhagat

Source: Ruminant Science (2013)-2(2):131-137.

How to cite this manuscript: AI Dadawala, BS Chandel, HC Chauhan and AG Bhagat (2013). Molecular detection of bovine rotavirus from north Gujarat, India. Ruminant Science 2(2):131-137.

Abstract

Seventy six faecal samples were collected from diarrhoeic bovine calves up to 4 weeks of age to find out the prevalence of rotavirus associated with neonatal calf diarrhoea. All 76 samples were processed for detection of rotavirus antigen by LAT and rotavirus genome by AGE and RT-PCR. All 76 faecal samples were processed for detection of rotavirus antigens by using latex agglutination test, while agar gel electrophoresis and RT-PCR was employed for the detection of rotavirus genome. Of these, 14 (18.42%), 6(7.89%) and 6(7.89%) were found positive in LAT, AGE and RT-PCR, respectively.

References

Al-Yousif Y, Anderson J, Chard-Bergstrom C, Bustamante A, Muenzenberger M, Austin K and  Kapil S (2001). Evaluation of a latex agglutination kit (Virogen Rotatest) for detection of bovine rotavirus in fecal samples. Clinical and Diagnostic Laboratory Immunology 8 (3):496-498.

Asano KM, Souza SP de, Barros IN de, Ayres GR, Silva SOS, Richtzenhain LJ and Brandão PE (2010). Multiplex semi-nested RT-PCR with exogenous internal control for simultaneous detection of bovine coronavirus and group A rotavirus. Journal of Virological Methods 169(2):375-379.

Basera SS, Singh R, Vaid N, Sharma  K, Chakrabarti S and Malik YPS (2010). Detection of Rotavirus infection in bovine calves by RNA-PAGE and RT-PCR. Indian Journal of Virology 21(2):144-147.

Bukrinskaia AG, Sharova NK, Sergeev OV, Vasil’ev Bla and Ten NL (1990). The RNA electrophoretypes of the rotaviruses circulating in Moscow and Leningrad in the winter of 1987-1988. Voprosy Virusologii 35(3):216-218.

Cho YI, Kim WI, Liu S, Kinyon JM and Yoon KJ (2010). Development of a panel of multiplex real-time polymerase chain reaction assays for simultaneous detection of major agents causing calf diarrhoea in faeces. Journal of Veterinary Diagnostic Investigation 22(4):509-17.

Chomczynski P and Mackey K (1995). Modification of the TRIZOL reagent procedure for isolation of RNA from polysaccharide-and proteoglycan-rich sources. Biotechniques 19(6):942-5.

Estes MK and Cohen J (1989). Rotavirus gene structure and function. Microbiological Reviews 53(4):410-449.

Goto Y, Kurogi H, Inaba Y and Matumoto M (1986). Sequential isolation of rotavirus from individual calves. Veterinary Microbiology 11(1-2):177-184.

Gulati BR, Maherchandani S and Pandey R (1995). Electropherotypic typing of the genomic RNA of rotavirus from diarrhoeic calves. Indian Journal of Virology 11:7-12.

Herbst W, Lange H, Zschöck M and Krauss H (1986). Detection of calf rotavirus in the rotascreen latex test and electron microscopy-A comparative study. Dtsch Tierarztl Wochenschr  93 (7):317-319.

Isegawa Y, Nakagomi O, Nakagomi T, Ishida S, Uesugi S and Ueda S (1993). Determination of bovine rotavirus G and P serotype by polymerase chain reaction. Molecular Cell Probes 7:277-284

Matthijnssens J (2012). Genotype constellation of epidemiologically important human rotaviruses. Compendium 10th International Rotavirus Symposium, Bangkok, Thailand, September 19-21.

Kalyani IH (1997). Detection, propagation, experimental infection and electropherogram studies of bovine rotavirus. MVSc thesis submitted to Gujarat Agricultural University, Anand, Gujarat.

Kapadia DK, Joshi DV, Chaudhary SS, Shah NM, Panchasara HH and Patel BJ (2012a). Diagnosis of rotavirus infection in neonatal calves by ribos nucleic acid polyacrylamide electrophoresis (RNA-PAGE) and double antibody sandwich enzyme linked immunosorbant assay (DAS-ELISA). Ruminant Science 1(1):15-18.

Kapadia DK, Joshi DV, Shah NM and Panchasara HH (2012b). Serotyping and in vitro drug sensitivity of Escherichia coli isolated from neonatal buffalo calves diarrhoea. Ruminant Science 1(2):141-143.

Manuja BK, Prasad M, Manuja A, Gulati BR and Prasad G (2008). A novel genomic constellation (G10P[3]) of group A rotavirus detected from buffalo calves in northern India. Virus Research 138(1-2):36-42.

Mathur MS and Bhave GG (1993). Comparative evaluation of coagglutination and latex agglutination test (Rotalex kit) for detection of rotavirus. Journal of Postgraduate Medicine 39(3):130-131.

Minakshi,  Pandey R, Prasad G and  Malik Y (2001). VP4 gene specific RT-PCR for detection of buffalo group A rotaviruses. Indian Journal of Animal Science 71:611-613.

Niture GS, Karpe AG, Prasad Minakshi, Bhonsle AV and Ingale SS (2009). Genomic Diversity among Rotaviruses isolated from Diarrhoeic Buffalo calves. Veterinary World 2(7):259-260

Pandey A and Pandey R (1999). Compression between RNA electropherotyping and nucleic acid hybridization for the detection of rotavirus in faecal samples of diarrhoeic calves. Indian Journal of Animal Science 68:595-94.

Panja P, Saerkar S, Chakraborty M, Das G and Chowdhary A (2001). Detection of group and subgroup specific antigens of bovine rotaviruses from diarrhoeic calves in West Bengal. Indian Journal of Animal Health 40(2):129-132.

Quinn PJ, Markey BK, Leonard FC, Fitzpatrick ES, Fanning S and Hartigan PJ (2011). In: Veterinary Microbiology and Microbial Disease. 2nd Edn, Blackwell Science Ltd.

Rodríguez-Díaz J, Rubilar-Abreu E, Spitzner M, Hedlund KO, Liprandi F and Svensson L (2008). Design of a multiplex nested PCR for genotyping of the NSP4 from group a rotavirus. Journal of Virological Methods 149:240-245.

Samuel Jakobsson (2013). Diagnosis and molecular epidemiology of bovine rotavirus and coronavirus in brazil. Degree project, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences.

Shah NM (1989). Microbiological investigations of neonatal calf diarrhoea with specific reference to detection of rota virus and enterotoxigenic E. coli. PhD thesis submitted to Gujarat Agaricultural University, Sardar Krushinagar, Gujarat.

Singh TC and Jhala MK (2011). G-Typing of Bovine Rotaviruses by using Vp7 Gene specific heminested  RT-PCR from diarrhoeic calf faecal samples. Buffalo Bulletin 30(2):113-120.

Snodgrass DR (1986). Evaluation of a combined rotavirus and enterotoxigenic Escherichia coli vaccine in cattle. Veterinary Record 119(2):39-42.

Suelen Paesi, Caciano Elonir da Rosa, Phelipe Rodrigues Marocco Dornelles, Felipe da Luz, Cesar Augusto  Schenkel and  Denise Zampieri (2012). Evaluation of a latex agglutination kit for detecting rotavirus in Piglets. Acta Scientiae Veterinariae 40(1):1014.

Suresh T, Rai RB, Dhama K, Bhatt P, Sawant PM and Sharma AK (2011). Detection of group A bovine rotavirus in diarrhoeic calves by reverse transcriptase polymerase chain reaction (RT-PCR) and electropherotyping. Veterinary Practitioner 12(2):133-137.

Swiatek DL, Palombo EA, Lee A, Coventry MJ, Britz ML and Kirkwood CD (2010). Detection and analysis of bovine rotavirus strains circulating in Australian calves during 2004 and 2005. Veterinary Microbiology 140(1-2):56-62.

Torsein M, Lindberg A, Sandgren CH, Waller KP, Törnquist M and Svensson C (2010). Risk factors for calf mortality in large Swedish dairy herds. Preventive Veterinary Medicine 99(2-4):136-47.

Xu L, Harbour D and McCrae MA (1990). The application of polymerase chain reaction to the detection of rotaviruses in faeces.  Journal of Virological Methods 27(1):29-37.

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3-Title: Clinico-pathology of sheep pox disease in Himachal Pradesh, India

Authors: Rinku Sharma, RD Patil, HA Parimoo, D Thakur and VC Katoch

Source: Ruminant Science (2013)-2(2):127-130.

How to cite this manuscript: Sharma Rinku, Patil RD, Parimoo HA, Thakur D and Katoch VC (2013). Clinico-pathology of sheep pox disease in Himachal Pradesh, India. Ruminant Science 2(2):127-130.

Abstract

This communication presents the clinico-epidemiological observations and detailed gross and histopathology of sheep pox disease outbreak in Himachal Pradesh state of India. Grossly, multisystemic nodular lesions, mucopurulent nasal discharges and respiratory distress were observed in the affected animals. Specific histopathological features like hyperplasia of epithelial cells, hydropic degeneration and sheep pox cells with presence of intracytoplasmic eosinophilic inclusion bodies around blood vessels in dermis of skin were observed. The disease was confirmed on the basis of characteristic gross pathology and pathognomonic histopathological lesions. Sheep pox is an emerging viral disease in the region and efforts need to be diverted towards its control, thereby preventing economic losses to the farmers.

References

Bhanuprakash V, Indrani BK, Hegde R, Kumar MM and Moorthy ARS (2004). A classical live attenuated vaccine for sheep pox. Tropical Animal Health and Production 36:307-320.

Bhanuprakash V, Indrani BK, Hosamani M and Singh RK (2006). The current status of sheep pox disease. Comparative Immunology Microbiology and Infectious Diseases 29:27-60.

Diallo A and Viljoen GJ (2007). Genus Capripoxvirus. In: Poxviruses. Eds: AA Mercer, A Schmidt and O Weber. Birkhauser, Basel, Switzerland. pp 167-181.

Eroksuz Y, Bulut H, Gulacti I and Ceribasi AO (2008). Seasonal distribution of sheep pox cases in lambs in Eastern Turkey. Journal of Animal and Veterinary Advances 7:638-642.

Garner MG, Sawarkar SD, Brett EK, Edwards JR, Kulkarni VB, Boyle DB and Singh SN (2000). The extent and impact of sheep pox and goat pox in the state of Maharashtra, India. Tropical Animal Health and Production 32:205-223.

Hailat N, Al-Rawashdeh O, Lafi S and Al-Bateineh Z (1994). An outbreak of sheep pox associated with unusual winter conditions in Jordan. Tropical Animal Health and Production 26:79-80.

Verma S, Verma LK, Gupta VK, Katoch VC, Dogra V, Pal B and Sharma M (2011). Emerging capripoxvirus disease outbreaks in Himachal Pradesh, a northern state of India. Transboundary and Emerging Diseases 58:79-85.

Yashpal M, Mahesh K, Batra SK, Chand P, Malik Y and Khatri M (1997). Occurrence of sheep pox in unorganized flocks in Haryana State. Indian Journal of Animal Science 67:962-963.

Kitching RP and Taylor WP (1985). Transmission of capripoxvirus. Research in Veterinary Science 39:196-199.

Luna LG (1968). Manual of Histological Staining Methods of the Armed Forces Institute of Pathology. 3rd Edn, McGraw Hill Book Co, New York.

Murthy DK and Singh PP (1971). Epidemiological studies on an outbreak of sheep pox in a mixed flock in Uttar Pradesh. Indian Journal of Animal Science 41:1072-1079.

Tulman ER, Afonsof CL, Lu Z, Zszk L, Sur JH, Sandybaev NT, Kerembekova UZ, Zaitsev VL, Kutish GF and Rock DL (2002). The genomes of sheep pox and goat pox viruses. Journal of Virology 76:6054-6061.

2

2-Title: Experimental study to control the abscess disease in Najdi sheep at Qassim region of Saudi Arabia

Authors: KB Al-Harbi, MA Al-Dubaib and OM Mahmoud

Source: Ruminant Science (2013)-2(2):123-126.

How to cite this manuscript: Al-Harbi KB, Al-Dubaib MA and Mahmoud OM (2013). Experimental study to control the abscess disease in Najdi sheep at Qassim region of Saudi Arabia.  Ruminant Science 2(2):123-126.

Abstract

A program for controlling the abscess disease of sheep, based on vaccination, zinc injection and antiseptic washing, was implemented on a sheep flock comprising 50 Najdi ewes (2-3 years) and 3 breeding rams. The animals in the flock were vaccinated with a bacterin (GlanvacTM) and washed with Dettol antiseptic at its standard dilution (1/125) every 6 months. They were also injected subcutaneously with 5 mg/kg bodyweight zinc as zinc oxide suspended in olive oil, once annually. The ewes in the main flock were allowed to breed freely and the born F1 generation of lambs (n=20) joined the control program at the age of 3-4 months. The ewes and the lambs were monitored for abscess development and for general health for two years. In the first year, two vaccinated ewes developed abscesses on the head with incidence of 3.8% (2/53). Two non-vaccinated lambs developed abscesses at the age of 3 months before joining the control program. The program was continued on the parent stock ewes and their F1 generation lambs for a second year. The incidence of abscesses was 0% for both dams and lambs in the second year.  The F1 generation reached maturity and was bred to produce F2 generation of lambs (n=8) that also joined the control grogram at the age of 3-4 months. The F2 generation of lambs remained free of abscesses until the age of 5 months, the time when the experiment time expired. The results show that the program is highly effective in the control of sheep abscess disease as from the second year of its implementation.

References

Al-Harbi KB (2011). Prevalence and etiology of abscess disease in sheep and goats at Qassim region, Saudi Arabia. Veterinary World 4(11):495-499.

Baird GJ and Malone FE (2010). Control of caseous lymphadenitis in six sheep flocks using clinical examination and regular ELISA testing. Veterinary Record 166:358-292.

Dercksen DF, Brinkhof JMA, Dekker-Nooren T, Maanen K, Bode CF, Baird G and Kamp EM (2000). A comparison of four serological tests for the diagnosis of caseous lymphadenitis in sheep and goats. Veterinary Microbiology 75:167-175.

Dorella FA, Pacheco LG, Seyffert N, Portela RW, Meyer R, Miyoshi A and Azevedo V (2009). Antigens of Corynebacterium psuedotuberculosis and prospectus for vaccine development. Expert Review in Vaccines 8:205-213.

Fontaine MC, Baird G, Conner KM, Rudge K, Sales J and Donachie W (2006). Vaccination confers significant protection of sheep against infection with a virulent United Kingdom strain of C. psuedotuberculosis. Vaccine 14:33-34.

Hambridge KM, Casey CE and Krebs J (1986). Zinc. In: Trace Elements in Man and Animals Nutrition. 5th Edn, Volume 2, Academic Press, Orlando. pp 1-37.

Ibs KH and Rink L (2003). Zinc-altered immune function. Journal of Nutrition 133:1452S-1456S.

Ivanovic S, Zutic I, Pavlovic I and Zujovic M (2009). Caseous lymphadenitis in goats. Biotechnology in Animals 25:999-1007.

Paton MW, Mercy AR, Sutherland SS, Ellis TM and Duta SR (1991). The effect of antibody to caseous lymphadenitis in ewes on the efficacy of vaccination in lambs. Australian Veterinary Journal 68 (4):143-146.

Underwood EJ (1981). Mineral Nutrition of Farm Animals. Common Agricultural Bureaux, UK.

Williamson LH (2001). Caseous lymphadenitis in small ruminants. Veterinary Clinics of North America: Food Animal Practice 17:359-371.

Wu FYH and Wu CH (1987). Zinc in DNA replication and transcription. Annual Review of Nutrition 7:251-272.