Partial BLV RNAs are present in plasma exosomes of BLV infected animals

1.Title: Partial BLV RNAs are present in plasma exosomes of BLV infected animals

Authors: Marawan A Marawan, Abdel-moneim M Moustafa, Faysal K Arnaout and El Sayed M Galila

Source: Ruminant Science (2016)-5(2):133-142.

Cite this reference as: Marawan Marawan A, Moustafa Abdel-moneim M, Arnaout Faysal K and Galila El Sayed M (2016). Partial BLV RNAs are present in plasma exosomes of BLV infected animals. Ruminant Science 5(2):133-142.


Exosomes are membranous nano-vesicles of endocytic origin that are released by almost all cell types and play an important role in cell-to-cell communication. Many researches have been carried out to illustrate the role of exosomes in the life cycle and pathogenesis of RNA viruses, however, information regarding their role in Bovine leukemia virus (BLV) is meagre. Herein we tried to investigate the role of exosomes isolated from plasma in BLV life cycle. Exosomal fractions were isolated from 18 fresh BLV-positive plasma samples by size exclusion chromatography (SEC) and then were tested using western blotting, RT-PCR and sequence analysis. Our results showed that absence of BLV enveloped protein (env) in all tested exosomal fractions, on the other hand around 22% of samples showed the presence of partial BLV RNA in their exosomes. From this approach, it was emphasized that the exosomes may incriminate in BLV pathogenesis through transfer of viral RNA from infected to uninfected B cells.



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