Partial BLV RNAs are present in plasma exosomes of BLV infected animals

1.Title: Partial BLV RNAs are present in plasma exosomes of BLV infected animals

Authors: Marawan A Marawan, Abdel-moneim M Moustafa, Faysal K Arnaout and El Sayed M Galila

Source: Ruminant Science (2016)-5(2):133-142.

Cite this reference as: Marawan Marawan A, Moustafa Abdel-moneim M, Arnaout Faysal K and Galila El Sayed M (2016). Partial BLV RNAs are present in plasma exosomes of BLV infected animals. Ruminant Science 5(2):133-142.

Abstract

Exosomes are membranous nano-vesicles of endocytic origin that are released by almost all cell types and play an important role in cell-to-cell communication. Many researches have been carried out to illustrate the role of exosomes in the life cycle and pathogenesis of RNA viruses, however, information regarding their role in Bovine leukemia virus (BLV) is meagre. Herein we tried to investigate the role of exosomes isolated from plasma in BLV life cycle. Exosomal fractions were isolated from 18 fresh BLV-positive plasma samples by size exclusion chromatography (SEC) and then were tested using western blotting, RT-PCR and sequence analysis. Our results showed that absence of BLV enveloped protein (env) in all tested exosomal fractions, on the other hand around 22% of samples showed the presence of partial BLV RNA in their exosomes. From this approach, it was emphasized that the exosomes may incriminate in BLV pathogenesis through transfer of viral RNA from infected to uninfected B cells.

 

References

An T, Qin S, Xu Y, Tang Y, Huang Y, Situ B, Inal JM and Zheng L (2015). Exosomes serve as tumour markers for personalized diagnostics owing to their important role in cancer metastasis. Journal of Extracellular Vesicles 4.

Asfaw Y, Tsuduku S, Konishi M, Murakami K, Tsuboi T, Wu D and Sentsui H (2005). Distribution and superinfection of bovine leukemia virus genotypes in Japan. Archives of Virology 150:493-505.

Balic D, Lojkic I, Periskic M, Bedekovic T, Jungic A, Lemo N, Roic B, Cac Z, Barbic L and Madic J (2012). Identification of a new genotype of bovine leukemia virus. Archives of Virology 157:1281-1290.

Bartlett PC, Sordillo LM, Byrem TM, Norby B, Grooms DL, Swenson CL, Zalucha J and Erskine RJ (2014). Options for the control of bovine leukemia virus in dairy cattle. Journal of the American Veterinary Medical Association 244:914-922.

Bukong TN, Momen-Heravi F, Kodys K, Bala S and Szabo G (2014). Exosomes from hepatitis C infected patients transmit HCV infection and contain replication competent viral RNA in complex with Ago2-miR122-HSP90. PLoS Pathog 10: e1004424.

Chahar HS, Bao X and Casola A (2015). Exosomes and their role in the life cycle and pathogenesis of RNA viruses. Viruses 7:3204-3225.

Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM and Falcon-Perez JM (2008). Characterization and comprehensive proteome profiling of exosomes secreted by hepatocytes. Journal of Proteome Research 7:5157-5166.

Durkin K, Rosewick N, Artesi M, Hahaut V, Griebel P, Arsic N, Burny A, Georges M and Van den Broeke A (2016). Characterization of novel Bovine Leukemia Virus (BLV) antisense transcripts by deep sequencing reveals constitutive expression in tumors and transcriptional interaction with viral microRNAs. Retrovirology 13:1.

Gillet NA, Gutierrez G, Rodriguez SM, de Brogniez A, Renotte N, Alvarez I, Trono K and Willems L (2013). Massive depletion of bovine leukemia virus proviral clones located in genomic transcriptionally active sites during primary infection. PLoS Pathogens 9:e1003687.

Jaworski E, Narayanan A, Van Duyne R, Shabbeer-Meyering S, Iordanskiy S, Saifuddin M, Das R, Afonso PV, Sampey GC and Chung M (2014). Human T-lymphotropic virus type 1-infected cells secrete exosomes that contain Tax protein. Journal of Biological Chemistry 289:22284-22305.

Juliarena MA, Lendez PA, Gutierrez SE, Forletti A, Rensetti DE and Ceriani MC (2013). Partial molecular characterization of different proviral strains of bovine leukemia virus. Archives of Virology 158:63-70.

Kettmann R, Marbaix G, Cleuter Y, Portetelle D, Mammerickx M and Burny A (1980). Genomic integration of bovine leukemia provirus and lack of viral RNA expression in the target cells of cattle with different responses to BLV infection. Leukemia Research 4:509-519.

Kim YH, Lee EY, Oem JK, Kim SH, Lee MH, Lee KK and Park SC (2015). Genetic analysis of env and gag gene fragments of bovine leukemia virus identified in cattle from Korea. Korean Journal of Veterinary Research 55:53-56.

Lin J, Li J, Huang B, Liu J, Chen X, Chen X-M, Xu Y-M, Huang L-F and Wang X-Z (2015). Exosomes: novel biomarkers for clinical diagnosis. The Scientific World Journal 2015.

Mathivanan S, Fahner CJ, Reid GE and Simpson RJ (2012). ExoCarta 2012: database of exosomal proteins, RNA and lipids. Nucleic Acids Research 40:D1241-D1244.

Mekata H, Sekiguchi S, Konnai S, Kirino Y, Honkawa K, Nonaka N, Horii Y and Norimine J (2015). Evaluation of the natural perinatal transmission of bovine leukaemia virus. Veterinary Record 176:254-254.

Narayanan A, Iordanskiy S, Das R, Van Duyne R, Santos S, Jaworski E, Guendel I, Sampey G, Dalby E and Iglesias-Ussel M (2013). Exosomes derived from HIV-1-infected cells contain trans-activation response element RNA. Journal of Biological Chemistry 288:20014-20033.

Nekouei O, Stryhn H, VanLeeuwen J, Kelton D, Hanna P and Keefe G (2015). Predicting within-herd prevalence of infection with bovine leukemia virus using bulk-tank milk antibody levels. Preventive Veterinary Medicine.

Nordin JZ, Lee Y, Vader P, Mäger I, Johansson HJ, Heusermann W, Wiklander OP, Hällbrink M, Seow Y and Bultema JJ (2015). Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties. Nanomedicine: Nanotechnology, Biology and Medicine 11:879-883.

Ohno A, Takeshima SN, Matsumoto Y and Aida Y (2015). Risk factors associated with increased bovine leukemia virus proviral load in infected cattle in Japan from 2012 to 2014. Virus Research 210:283-290.

Sevik M, Avcı O and İnce ÖB (2015). An 8-year longitudinal sero-epidemiological study of bovine leukaemia virus (BLV) infection in dairy cattle in Turkey and analysis of risk factors associated with BLV seropositivity. Tropical Animal Health and Production 47:715-720.

Sharma A, Khatun Z and Shiras A (2016). Tumor exosomes: cellular postmen of cancer diagnosis and personalized therapy. Nanomedicine 11:421-437.

Subra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B and Silvente-Poirot S (2010). Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins. Journal of Lipid Research 51:2105-2120.

Taylor DD, Zacharias W and Gercel-Taylor C (2011). Exosome isolation for proteomic analyses and RNA profiling. Serum/Plasma Proteomics: Methods and Protocols:235-246.

van Niel G, Porto-Carreiro I, Simoes S and Raposo G (2006). Exosomes: a common pathway for a specialized function. Journal of Biochemistry 140:13-21.

Vojtech L, Woo S, Hughes S, Levy C, Ballweber L, Sauteraud RP, Strobl J, Westerberg K, Gottardo R and Tewari M (2014). Exosomes in human semen carry a distinctive repertoire of small non-coding RNAs with potential regulatory functions. Nucleic Acids Research 347.

Welton JL, Webber JP, Botos L-A, Jones M and Clayton A (2015). Ready-made chromatography columns for extracellular vesicle isolation from plasma. Journal of Extracellular Vesicles 4.

Yamada T, Shigemura H, Ishiguro N and Inoshima Y (2013). Cell Infectivity in relation to bovine leukemia virus gp51 and p24 in bovine milk exosomes. PloS one 8:e77359.

Zech D, Rana S, Büchler MW and Zöller M (2012). Tumor-exosomes and leukocyte activation: an ambivalent crosstalk. Cell Communication and Signaling 10:1.

Zomer A, Vendrig T, Hopmans ES, van Eijndhoven M, Middeldorp JM and Pegtel DM (2010). Exosomes: fit to deliver small RNA. Communicative and Integrative Biology 3:447-450.