Association of genetic variation at intronic region of bovine brca1 gene with occurrence of mastitis in HF crossbred cattle
Title: Association of genetic variation at intronic region of bovine brca1 gene with occurrence of mastitis in HF crossbred cattle
Authors: Shankarappa Bhajantri, MR Jayashankar, R Nagaraja, YB Rajeshwari, A Krishnaswamy, SB Prasanna, S Naveen Kumar and HMYathish
Source: Ruminant Science (2018)-7(1):37-41.
Cite this reference as: Bhajantri Shankarappa, Jayashankar MR, Nagaraja R, Rajeshwari YB, Krishnaswamy A, Prasanna SB, Kumar SN and Yathish HM (2018). Association of genetic variation at intronic region of bovine brca1 gene with occurrence of mastitis in HF crossbred cattle. Ruminant Science 7(1):37-41.
Breast cancer 1, early onset gene (BRCA1) is one of the most potent genes located within or nearby the genomic region of somatic cell score (SCS) quantitative trait loci (QTL) and is considered as a candidate gene for disease resistance. The present study was carried out to explore the polymorphism in intronic region of BRCA1 gene and its association with mastitis in HF crossbred cows using PCR-RFLP. Milk and blood samples were collected from 152 unrelated lactating HF crossbred cows. Milk samples were subjected to California mastitis test (CMT), electrical conductivity (EC) and somatic cell count (SCC) tests. The animals were classified into mastitis free (52) and mastitis affected (100) groups mainly based on SCC because of its higher sensitivity and specificity as compared to CMT and EC. The PCR-RFLP analysis of intron 6 region (321bp) of BRCA1 gene revealed the existance of genetic variation, where the frequencies of AA, BB and AB, genotypes were 0.21, 0.17 and 0.62, respectively and the frequencies of A and B alleles were 0.51 and 0.49, respectively. Sequencing of representative samples for each genotype confirmed the presence of 321 bp nucleotides conforming to the intron 6 region of BRCA1 gene and revealed no nucleotide changes in HhaI restriction sites in ‘A’ allele. Alignment of ‘A’ and ‘B’ allele sequences revealed G>A transition at 272nd and C>A transversion at 273rd positions in ‘B’ allele. The chi-square analysis revealed the deviation of HF crossbred population from HWE. Further, chi-square analysis did not reveal any significant association between BRCA1 genotypes and mastitis.
Daetwyler HD, Schenkel FS, Sargolzaei M and Robinson JA (2008). A genome scan to detect quantitative trait loci for economically important traits in Holstein cattle using two methods and a dense single nucleotide polymorphism map. Journal of Dairy Science 91:3225.
Deb R, Singh U, Kumar S, Singh R, Sengar G and Sharma A (2014). Profiling of bovine breast cancer 1, early onset (BRCA1) gene among Frieswal (HF × Sahiwal) cows and their association with mastitis. National Academy Science Letters 37(6):579-583.
Falconer DS and Mackay TFC (1996). Introduction to Quantitative Genetics. 4th Edn, Longman group Ltd. Essex. England. FAG Production Year Book, Vol. 53.
Hegde R, Shrikrishna Isloor, Nithin Prabhu K, Shome B R, Rathnamma D, Suryanarayana VS, Yatiraj S, Renuka Prasad C, Krishnaveni N, Sundareshan S, Akhila DS, Gomes AR and Nagendra RH (2012). Incidence of subclinical mastitis and prevalence of major mastitis pathogens in organized farms and unorganized sectors. Indian Journal of Microbiology 53(3):315-320.
Krum SA, Womack JE and Lane TF (2003). Bovine BRCA1 shows classic responses to genotoxic stress but low in vitro transcriptional activation activity. Oncogene 22:6032-6044.
Magotra A, Gupta ID, Verma A, Alex R, Vijaykumar, Vineeth MR, Arya A and Sakthivel Selvan A (2016a). Characterization and validation of point mutation in breast cancer 1 (BRCA1) and its relationship with mastitis traits in Sahiwal. International Journal of Recent Research in Interdisciplinary Sciences 3(1):22-26.
Magotra A, Gupta ID, Verma A, Alex R, Vineeth MR, Arya A Vijaykumar and Tiwari DK (2016b). Genetic profiling and validation of point mutation in exon 10 of breast cancer 1 gene (BRCA1) and its relationship with clinical mastitis in Sahiwal cattle. Ruminant Science 5(1):1-4.
Miller SA, Dykes DD and Polesky HF (1988). A sample salting out procedure for extraction of DNA from human nucleated cells. Nucleic Acids Research 16:1215.
Muhasin AVN, Bhushan B, Panigrahi M, Kumar A, Dewangan P, Gaur GK and Sharma D (2015). Lack of association of allelic variants of BRCA1 gene with mastitis susceptibility in Vrindavani cattle. Indian Journal of Animal Science 85(1):81-83.
Schalm OW and Noorlander DO (1957). Experiments and observations leading to the development of California mastitis test. Journal of American Veterinary Medical Association 130:199-204.
Schalm OW, Carroll EJ and Jain NC (1971). Bovine Mastitis. Lea and Febiger, Philadelphia.
Scully R and Livingston DM (2000). In search of the tumour-suppressor functions of BRCA1 and BRCA2. Nature 408(6811):429.
Shagufta F, Eram H, Hafsa N, Spozhmai B, Shanza L and Sidra L (2016). Determination of mastitis by measuring milk electrical conductivity. International Journal of Advanced Research in Biological Sciences 3(10):1-4.
Yuan Z, Chu G, Dan Y, Li J, Zhang L, Gao X, Gao H, Junya L, Xu S and Liu Z (2012). BRCA1: A new candidate gene for bovine mastitis and its association analysis between single nucleotide polymorphisms and milk somatic cell score. Molecular Biology Reports 39:6625-6631.
Yuan ZR, Li J, Liu L, Zhang LP, Zhang LM, Chen C, Chen XJ, Gao X, Li JY, Chen JB, Gao HJ and Xu SZ (2011). Single nucleotide polymorphism of CACNA2D1 gene and its association with milk somatic cell score in cattle. Molecular Biology Reports 38: 5179-5183.