Comparative evaluation of acrosomal integrity of bull spermatozoa by two staining techniques
Title: Comparative evaluation of acrosomal integrity of bull spermatozoa by two staining techniques
Authors: Nadeem Shah, HP Yadav, RK Gupta, DS Chauhan, Vijay Singh, Sarvajeet Yadav and DK Swain
Source: Ruminant Science (2016)-5(2):253-256.
Cite this reference as: Shah Nadeem, Yadav HP, Gupta RK, Chauhan DS, Singh Vijay, Yadav Sarvajeet and Swain DK (2016). Comparative evaluation of acrosomal integrity of bull spermatozoa by two staining techniques. Ruminant Science 5(2):253-256.
Present study evaluated the variations existing between the techniques of evaluation of acrosomal integrity in bull spermatozoa. Acrosomal integrity was evaluated in forty ejaculates collected from four Haraina bulls using traditional Giemsa staining (n=20) and fluorescent FITC-PSA labeling (n=20). Evaluation of intact acrosome and damaged acrosome
followed by different types of acrosomal damage (primary, secondary and tertiary) were carried out using these techniques. Results revealed that FITC-PSA labeling technique is better as compared to Giemsa in evaluation of intact as well different types of acrosomal damage. Simultaneously, we noted significantly better analysis of acrosomal intactness on Poly-L-Lysine coated slides as compared to general glass slides. The ease of preparation of smears and their evaluation was better on Poly-L-Lysine coated slides as compared to general glass slides. From the study, it was concluded that, evaluation of spermatozoa for identification of different types of acrosomal damage may be preferred to be evaluated by FITC-PSA as compared to Giemsa and Poly-L-Lysine coated slides may be preferred for better analysis of results. The study is beneficial to the scientific community in terms of staining as well as better interpretation of acrosomal status in animals.
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