Effect of sericin supplementation on the semen quality of cryopreserved hariana bull semen
Title: Effect of sericin supplementation on the semen quality of cryopreserved hariana bull semen
Authors: Chhote Lal Yadava, Atul Saxena, Brijesh Yadav, Vipin Singh, A Vidhya Sagar Reddy, Akhil Patel, Anuj Kumar and Sarvajeet Yadav
Source: Ruminant Science (2018)-7(1):93-96.
Cite this reference as: Yadava Chhote Lal, Saxena Atul, Yadav Brijesh, Singh Vipin, Reddy A Vidhya Sagar, Patel Akhil, Kumar Anuj and Yadav Sarvajeet (2018). Effect of sericin supplementation on the semen quality of cryopreserved hariana bull semen. Ruminant Science 7(1):93-96.
The present study was aimed to evaluate the beneficial effects of supplementation of sericin on seminal attributes during different stages of semen cryopreservation in Hariana bull. Twenty four ejaculates from four Hariana bulls were collected using artificial vagina and after initial examination the qualified ejaculates were divided into three aliquots; one aliquot was extended with egg yolk tris citrate (EYTG) extender (Control), second aliquot was extended with EYTG supplemented with sericin @ 0.25% (T1) and the third aliquot was extended with EYTG supplemented with Sericin @ 0.50% (T2). Samples were equilibrated and then cryo-preserved using programmable biological freezer in liquid nitrogen. Semen evaluation after equilibration (pre-freeze) and thawing revealed a significantly higher (p<0.05) per cent progressive motility, viability, spermatozoa with intact acrosome and HOS positive spermatozoa as compared to control. A significant (p<0.05) beneficial effect on different seminal attributes was also recorded in T1 as compared to T2 in equilibrated and post-thaw semen. It can be concluded that Sericin @0.25% supplementation to the egg yolk tris citrate (EYTG) extender provided better cryo-protective effect on equilibrated and post-thaw Hariana semen.
Bailey JL, Bilodeau JF and Cormier N (2000). Semen cryopreservation in domestic animals: A damaging and capacitating phenomenon. Journal of Andrology 21:1-7.
Bloom E (1950). A one minute live-dead sperm stain by means of eosin-nigrosin. Fertility and Sterility 1:176-177.
Chatterjee S and Gagnon ( 2001). Production of reactive oxygen species by spermataozoa undergoing cooling, freezing, and thawing. Molecular Reproduction Development 59:451-458.
Curry MR (2000). Cryopreservation of semen from domestic livestock. Reviews in Reproduction 5:46-52.
Dash R, Acharya C, Bind PC and Kundu SC (2008). Antioxidant potentialof silk protein sericin against hydrogen peroxide-induced oxidativestress in skin fibroblasts. BMB Reproduction 41:236-241.
Farber JL, Kyle ME and Cleman JB (1990). Mechanism of cell injury by activated oxygen species. Lab Invest 62:670-679.
Hafez ESE (2000). Reproduction in Farm Animals. 7th Edn, pp 293-367
Hancock JL (1951). A staining technique for the study of temperature shock in semen. Nature 169:323-326.
Holt WV (2000a). Fundamental aspects of sperm cryobiology: The importance of species and individual differences. Theriogenology 53:47-58.
Holt WV (2000b). Basic aspects of frozen storage of semen. Animal Reproduction Science 62:3-22.
Jeyendran RS, Vander-Ven H, Perez-Pelaez M, Crabo BG and Zanevld LJD (1984). Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characters. Journal of Reproduction and Fertility 70:219-228.
Kato N, Sato S, Yamanaka A, Yamada H, Fuwa N and Nomura M (1998). Silk protein, sericin, inhibits lipid peroxidation and tyrosinase activity. Journal of Biotechnology and Applied Biochemistry 62:145-147.
Kumar P, Kumar D, Sikka P and Singh P (2015). Sericin supplementation improves semen freezability of buffalo bulls by minimizing oxidative stress during cryopreservation. Animal Reproduction Science 152:26-31.
Masakazu T, Kazuhisa T, Hideyuki Y, Hiroshi T and Shigeru N (2003). The silk protein, sericin, protects against cell death caused by acute serum deprivation in insect cell culture. Biotechnology Letter 25:1805-1809.
Mustapha AR, Beigh YA, Abhishek K, Rahul K, Omer D, Kumar A, Prasad JK, Bhure S, Srivastava N and Ghosh SK (2017). Effects of partial deoxygenation of extender on plasma membrane integrity and enzyme activity in frozen-thawed crossbred bull semen. Ruminant Science 6(2):327-331.
O’Flaherty C and Berlegni N (1997). Effect of natural antioxidants, superoxide demitasse and hyper oxide on capacitation of frozen thawed bull sperm. Andrologia 29:269-275.
Patel BR and Siddiquee GM (2013). Effect of semen additives on leakage of certain enzymes during cryopreservation of Kankrej semen. Ruminant Science 2(1):63-66.
Purdy PH (2006). A review on goat sperm cryopreservation. Journal of Small Ruminant Research 63(3):215-225.
Reddy VS, Yadav B, Yadav CL, Anand M, Swain DK, Kumar D, Kritania D, Madan AK, Kumar J and Yadav S (2018). Effect of sericin supplementation on heat shock protein 70 (HSP70) expression, redox status and post thaw semen quality in goat. Cryobiology 84:33-39.
Sahrawat AK, Saxena A and Mishra SK (2013). Cryopreservation of bhadawari buffalo bull spermatozoa in tris and citrate based extenders. Ruminant Science 2(1):67-70.
Sasaki M, Kato Y, Yamada H and Terada S (2005). Development of a novel serum free freezing medium for mammalian cells using the silk pro-tein sericin. Journal of Biotechnology and Applied Biochemistry 42:183-188.
Shukla MK, Misra AK and Gupta HP (2012). Caffeine as semen additive to improve murrah buffalo (Bubalus bubalis) semen cryopreservation. Ruminant Science 1(2):121-124.
Watson PF (1975). Use of Giemsa stain to detect changes in acrosomes of frozen ram spermatozoa. Veterinary Record 97:12-15.
Watson PF, Kunze E, Cramer P and Hammerstedt RH (1992). A comparison of critical osmolality and hydraulic conductivity and its activation energy in fowl and bull spermatozoa. Journal of Andrology 13(2):131-138.
Watson PF (2000). The causes of reduce fertility with cryopreserved semen. Animal Reproduction Science 60:481-492.