In-vitro maturation of buffalo oocytes IN TCM-199 media supplemented with follicular fluid, hormones and antioxidants
Title: In-vitro maturation of buffalo oocytes IN TCM-199 media supplemented with follicular fluid, hormones and antioxidants
Authors: AK Sharma, SS Chaudhary and Gopal Puri
Source: Ruminant Science (2017)-6(1):13-18.
Cite this reference as: Sharma AK, Chaudhary SS and Puri Gopal (2017). In-vitro maturation of buffalo oocytes in TCM-199 media supplemented with follicular fluid, hormones and antioxidants. Ruminant Science 6(1):13-18.
The aim of the present study was to evaluate the effects of follicular fluid, hormones and antioxidants (cysteamine and α-mercaptoethanol) in medium 199 for in vitro maturation of Surti and Mehsani breed buffalo oocytes. The culturable oocytes (1174) retrieved from buffalo ovaries by aspiration technique were used for in vitro maturation. Oocyte maturation rate was evaluated on the basis of visual assessment and degree of cumulus expansion after 24 hrs of in vitro culture at 38.5 ºC and 5% CO2 with 95 % relative humidity in CO2 incubator and were graded degree 0, 1 and 2 on the basis of cumulus expansion. Results of the study showed that the overall mean recovery rate of oocytes/ ovary was 2.20±0.08 while the culturable grade (Grade A and B combined) was 1.35±0.06, mean retrieval percentage of culturable COCs was 60.83±0.84. The percentage of matured oocytes for degree 2, 1 and 0 were 58.15±1.24, 21.99±0.70 and 19.86±1.19, respectively. The overall mean maturation percentage (degree 1 and 2) was 80.14±1.20. The present finding of experiment led to the conclusion that medium 199 supplemented with follicular fluid, hormones and antioxidants like cysteamine and α-mercaptoethanol @ 200mM each, improved the in vitro maturation rate of buffalo oocytes.
Adlak SA, Khillare KP, Pawshe CH and Mude SW (2008). Influence of commercially available follicle stimulating hormone on in vitro maturation of buffalo oocytes. Buffalo Bulletin 27:302-304.
Agrawal JK, Saxena Atul, Kharche SD, Pathak Juhi, Sikarwar AKS, Patel Akhil, Goel AK and Jindal SK (2016). A study on parthenogenetic activation and embryo production from in vitro matured caprine oocytes. Ruminant Science 5(1):21-24.
Alok P, Gupta SC and Neelam G (2010). Effect of FSH and LH hormones on oocyte maturation of buffalo and gene expression analysis of their receptors and Cx43 in maturing oocytes. Zygote 18(3):231-234.
Al-shimaa AH, El- Naby G, Karima HM, Mahmoud F, Youssef J, Ahmed E, Mahmoud A, Abouel R and Abdel-Ghaffar AE (2013). Effect of Season of the year and ovarian structures on oocytes recovery rate, quality and meiotic competence in Egyptian buffaloes. Global Veterinarian 10(4):408-412.
Barakat IAH, Kandeal SA, El-Ashmaoui HM, Barkawi A and EL-Nahass E (2012). Effect of medium type and luteinizing hormone (LH) on in vitro maturation of Egyptian buffalo oocytes. African Journal of Biotechnology 11(20):4620-4630.
Beheshti R, Mohammadi-Roshandeh A, Giasi Ghalehkandi J, Ghaemmaghami S and Houshangi AF (2011). Effect of antioxidant supplements on in vitro maturation of bovine oocyte. Advances in Environmental Biology 5(7):1473-1475.
Cevik M, Kocyigit A, Sen U and Kuran M (2014). Can sequential human embryo culture media be used in bovine in vitro embryo culture? Kafkas Universitesi veteriner Fakultesi Dergisi 20(1):149-153.
Chandra V, Mishra A and Sharma GT (2012). Effect of growth factors (epidermal growth factor, platelet derived growth factor, and insulin-like growth factor-1) on buffalo embryos produced in vitro. Indian Journal of Animal Sciences 82(12):1510-1514.
Chaudhary CF, Derashri HJ, Chaudhary SS and Puri G (2014). Quantitative and qualitative study of buffalo oocytes recovered by different oocyte harvesting techniques. Indian Journal of Animal Sciences 84(9):958-961.
Chauhan MS, Singla SK Palta P, Manik RS and Madan ML (1998). In vitro maturation and fertilization, and subsequent development of buffalo (Bubalus bubalis) embryos: Effects of oocyte quality and type of serum. Reproduction and Fertility Development 10:173-177.
De Matos DG, Gasparrini B, Pasqualini SR and Thompson JG (2002). Effect of glutathione synthesis stimulation during in vitro maturation of ovine oocytes on embryo development and intracellular peroxide content. Theriogenology 57:1443-1451.
Gabr SA, Khalek AEA and Ratel ITE (2015). Evaluation of some factors affecting quantity, quality and in vitro maturation of buffalo oocytes. Asian Journal of Animal and Veterinary Advances 10:1-13.
Gasparrini B, De Rosa A, Attanasio L, Boccia L, Di Palo R, Campanile G and Zicarelli L (2008). Influence of the duration of in vitro maturation and gamete co-incubation on the efficiency of in vitro embryo development in Italian Mediterranean buffalo (Bubalus bubalis). Animal Reproduction Science 105(3):354-364.
Gupta PSP, Nandi S, Raghu HM, Ravindranatha BM and Sarma PV (2003). Oocyte recovery rates in relation to morphology and weight of the ovaries in buffaloes. Indian Journal of Animal Sciences 37:147-148.
Hammam AM, Whisnant CS, Elias A, Zaabel SM, Hegab AO and Abu-El Naga EM (2010). Effect of media, sera and hormones on in vitro maturation and fertilization of water buffaloes (Bubalus bubalis). Journal of Animal and Veterinary Advances 9(1):27-31.
Hussain M, Barua PM, Biswas RK, Dutta D, Paul V, Chakravarty P, Deka BC and Sarmah BC (2012). In vitro maturation of indigenous cattle oocytes. Indian Veterinary Journal 89(2):59-62.
Jamil H, Samad HA, Qureshi ZI, Rehman NU and Lodhi LA (2008). Harvesting and evaluation of riverine buffalo follicular oocytes. Turkish Journal of Veterinary and Animal Sciences 32(1):25-30.
Kim M, Hong SJ, Lee JH, Min CK, Hwang KJ and Park RW (2011). Comparison of in vitro maturation media of immature oocytes: The effectiveness of blastocyst culture media. Fertility and Sterility 95:554-557.
Kobayashi K, Yamashitu S and Hushi H (1994). Influence of epidermal growth factors and transforming growth factors on in vitro maturation of cumulus enclosed bovine oocytes in defined medium. Journal of Reproduction and Fertility 12:439-446.
Kulasekhar T, Solmon K, Raju G and Sadasiva RK (2012). Efficacy of in-vitro maturation of bubaline oocytes in TCM 199, TCM 199 + PMSG and follicular fluid. Indian Journal of Animal Reproduction 33(1):33-36.
Kumar R, George A, Sharma R, Singla SK, Chauhan MS, Manik RS and Palta P (2013). Production of blastocysts from aggregates of buffalo embryonic stem cells and putative tetraploid buffalo embryos. Indian Journal of Animal Sciences 83(10):1033-1038.
Mahesh YU, Mutha RM, Sudhakar P and Sambasiva RKRS (2014). Effect of harvesting technique and presence or absence of corpus luteum on in vitro development after parthenogenetic activation of oocytes recovered from buffalo ovaries. Veterinary World 7(5):315-320.
Makwana PM and Shah RG (2009). Influence of different categories of follicle and presence of CL on recovery rate, quality and quantity of buffalo oocytes. Proceeding of XXV Annual convention of the ISSAR and international symposium, Veterinary College, Namakkal, Tamil Nadu, India, pp 286-287.
Mehmood A, Muhammad A, Andrabi SMH, Afzal M and Naqvi SMS (2011). In vitro maturation and fertilization of buffalo oocytes: The effect of recovery and maturation methods. Turkish Journal of Veterinary and Animal Sciences 35(6):381-386.
Patel PA, Chaudhary SS, Puri G, Singh VK and Odedara AB (2015). Effects of beta- mercaptoethanol on in vitro maturation and glutathione level of buffalo oocytes. Veterinary World 8(2):213-216.
Puri G, Chaudhary SS, Singh VK and Sharma AK (2015). Effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (Bubalis bubalis) oocytes in vitro. Veterinary World 8(2):143-146.
Raza A, Samad HA, Rehman NU and Zia EUH (2001). Studies on in vitro maturation and fertilization of Nili-Ravi buffalo follicular oocytes. International Journal of Agriculture and Biology 3(4):503-506.
Samad HA, Khan IQ, Rehmanb NU and Ahmed N (1998). The recovery, in vitro maturation and fertilization of Nilli-Ravi buffalo follicular oocytes. Asian Australian Journal of Animal Science 11:491-497.
Sanbuissho A and Threlfall WR (1990). The effect of serum and gonadotropins on in vitro maturation and fertilization of bovine oocytes. Theriogenology 34:341-348.
Sadeesh EM, Shah Fozia, Balhara AK, Thirumaran SMK, Balhara Sunesh and Yadav PS (2013). Effect of thiol compound supplemented culture medium on in vitro maturation of buffalo oocytes. Ruminant Science 2(1):59-62.
Sadeesh EM, Shah F, Balhara AK, Sumangali MK, Thirumaran K, Yadav S and Yadav PS 2014). Effect of growth factors and antioxidant on in vitro maturation of oocytes and cleavage rates of in vitro produced Indian buffalo (Bubalis bubalis) embryos. Veterinarski Archiv 84(5):459-474.
Singh B, Prasad S, Gupta HP and Prasad JK (2012). Effect of human active-A on in vitro maturation of oocytes and early embryonic development in buffaloes. Indian Journal of Animal Reproduction 33(2):67-72.
Singh V, Misra AK, Kumar S and Kumar V (2015). Effect of seasons on in-vitro maturation and cleavage of oocyte and in-vitro development of buffalo embryo. Ruminant Science 4(1):29-31.
Sabastian A, Nandi S, Kumar VG, Ummadahalli PS, Gowda S and Murthy VC (2013). Effect of sera of normal cycling, pregnant and repeat breeding buffaloes (Bubalis bubalis) on in vitro maturation of buffalo, sheep and goat oocytes. Asian Pacific Journal of Reproduction 2(2):110-113.
Sen U (2015). Effects of sperm from different bulls on developmental competence, blastocyst quality and cell number of bovine embryos in vitro. Kafkas University Veterinary Fakultesi Dergisi 21(3):339-344.
Singh S, Dhanda OP and Malik RK (2001). Effect of presence of corpus luteum on oocyte recovery and subsequent in vitro maturation and fertilization in buffaloes. Asian- Australian Journal of Animal Science 14(12):1675-1677.
Totey SM, Pawshe CH and Singh GP (1993). In vitro maturation and fertilization of buffalo oocytes (Bubalus Bubalis): Effects of media, hormones and sera. Theriogenology 39:1153-1171.
Wang ZG, Xu ZR and Yu SD (2007). Effects of oocyte collection techniques and maturation media on in vitro maturation and subsequent embryo development in Boer goat. Czech Journal of Animal Science 52:21-25.