MOLECULAR DETECTION OF Babesia bigemina FROM BLOOD OF DAIRY CATTLE
Title: MOLECULAR DETECTION OF Babesia bigemina FROM BLOOD OF DAIRY CATTLE
Authors: AB Parmar, HH Panchasara, KB Patel, HC Chauhan and BS Chandel
Source: Ruminant Science (2018)-7(2):287-288.
Cite this reference as: Parmar AB, Panchasara HH, Patel KB, Chauhan HC and Chandel BS (2018). Molecular detection of Babesia bigemina from blood of dairy cattle. Ruminant Science 7(2):287-288.
Babesiosis is a tick borne disease of exotic and crossbred animals having high morbidity, mortality and economic losses in livestock industries of India. In the present study a total 239 HF crossbred cattle were screened for babesiosis using PCR. Of the 33 clinically affected animals 14 samples were PCR positive (Group I- Clinical group) and 12 samples were positive out of 40 samples from cattle suspected for subclinical (Group II- sub-clinical group) Babesiosis using species specific primers The disease has prevalence rate of 10.87 percent based on polymerase chain reaction. A specific 1023 bp in gel electrophoresis indicate the Babesia bigemina species responsible for the babesiosis tick borne disease.
Azeemi TA, Zameer M and Rahman G (2013). Incidences of theileria and babesia in nangrahar province of Afghanistan. Ruminant Science 2(2):145-147.
Aziz KB, Khalil WKB, Mahmoud MS, Hassan NH, Mabrouk DM and Suarez CE (2014). Molecular characterization of babesiosis infected cattle: Improvement of diagnosis and profiling of the immune response genes expression. Global Veterinarian 12(2):197-206.
Criado-Fornelio A, Martinez-Marcos A, Buling- Sarana A and Barba-Carretero JC (2003). Molecular studies on babesia, theileria and hepatozoon in southern Europe. Veterinary Parasitology 114:173-194.
Faryal S, Khan K, Ali S and Akbar N (2015). Zoonotic significance and prophylactic measure against babesiosis. International Journal of Current Microbiology and Applied Sciences 4(7):938-953.
Figueroa JV, Chieves LP, Johnson GS and Buening GM (1992). Detection of Babesia bigemina infected carriers by polymerase chain reaction amplification. Journal of Clinical Microbiology 30:2578-2582.
Ke GM, Cheng HL, Ke LY, Ji WT, Chulu JLC, Liao MH, Chang TJ and Liu HJ (2006). Development of a quantitative light cycler real-time RT-PCR for detection of avian reovirus. Journal of Virology Methods 133:6-13.
Laha R, Mondal B, Biswas S, Chand K, Das M, Sarma D, Goswami A and Sen A (2015). Detection of Babesia bigemina infection in cattle from north-eastern India by polymerase chain reaction and its genetic relatedness with other isolates. Tropical Animal Health Production 47:633-636.
Radostits OM, Gay CC, Constable PD and Hinchcliff KW (2007). Veterinary Medicine. 10th Edn, WB Saunders Company, London (UK).
Sandra RT, Lina AG and Leonardo AR (2014). Assessing bovine babesiosis in Rhipicephalus (Boophilus) microplus ticks and 3 to 9-month-old cattle in the middle Magdalena region, Colombia. Veterinary Parasitology 34(4):313-319.
Silva JB, Andre MR, Fonseca AH, Lopes CT, Lima DH, Andrade SJ and Oliverira CM (2013). Molecular and serological prevalence of Babesia bovis and Babesia bigemina in water buffaloes in the north region of Brazil. Veterinary Parasitology 5:20.