A study on parthenogenetic activation and embryo production from in vitro matured caprine oocytes

A study on parthenogenetic activation and embryo production from in vitro matured caprine oocytes

Title: A study on parthenogenetic activation and embryo production from in vitro matured caprine oocytes

Authors: Jitendra Kumar Agrawal, Atul Saxena, SD Kharche, Juhi Pathak, AKS Sikarwar, Akhil Patel, AK Goel and SK Jindal

Source: Ruminant Science (2016)-5(1):21-24.

Cite this reference as: Agrawal Jitendra Kumar, Saxena Atul, Kharche SD, Pathak Juhi, Sikarwar AKS, Patel Akhil, Goel AK and Jindal SK (2016). A study on parthenogenetic activation and embryo production from in vitro matured caprine oocytes. Ruminant Science 5(1):21-24.

Abstract

The objective was to study the effectiveness of calcium ionophore treatment for activation of oocytes and theirdevelopmental capacity in vitro. A total of 5585 oocytes were retrieved from 1154 caprine ovaries of abattoir origin. Recovery rate of oocytes per ovary was recorded 4.84. Oocytes were matured in TCM-199 containing 10% FBS, sodium pyruvate 0.25 mM, L-glutamine 100 μg /ml, LH 5 μg/ml, FSH 5 μg/ml, BSA 3 mg/ml and gentamicin 50 μg /ml for 27 hours in humidified atmosphere of 5% CO2 at 38.50C in CO2 incubator. After parthenogenetic activation treatment using 5μm calcium ionophore for 5 min, oocytes were treated with 2mM DMAP for 3 hours and then cultured in embryo development media. The results suggested that the overall developmental rate through parthenogenetic activation by 5μm calcium ionophore for 5 min recorded was 56.02% (29.91%, 2-cell; 11.01%, 4-cell; 9.15%, 8-16 cell; 4.94%, morula; 1.01%, blastocysts) in vitro matured caprine oocytes.

References

Bing YZ, Che L, Hirao Y, Takenouchi N, Rodriguezmartinez H and  Nagai T (2003). Parthenogenetic activation and subsequent development of porcine oocytes activated by a combined electric pulse and butyrolactone I treatment. Journal of Reproduction and Development 49(2):159-166.

De AK, Malakar D, Jena MK, Dutta R, Garg S and Akshey YS (2012). Zona-free and with-zona parthenogenetic embryo production in goat (Capra hircus)-Effect of activation methods, culture systems and culture media. Livestock Science 143:35-42.

Ducibella T, Huneau D, Angelichio E, Xu Z, Schultz RM, Kopf GS, Fissore R, Madoux S and Ozil JP (2002). Egg-to-embryo transition is driven by differential responses to Ca2+ oscillation number. Developmental Biology 250:280-291.

Jellerette T, Melican D, Butler R, Nims S, Ziomek C, Fissore R and Gavin W (2006). Characterization of calcium oscillation patterns in caprine oocytes induced by IVF or an activation technique usedin nuclear transfer. Theriogenology 65:1575-1586.

Kharche SD, Goel AK, Jindal SK, Sinha NK and Yadav P (2008). Effect of somatic cells co-cuture on cleavage and development of in-vitro fertilized caprine embryos. Indian Journal of Animal Sciences 78:686-92.

Kharche SD and Birade H (2013). Parthenogenesis and activation of mammalian oocytes for in vitro embryo production: A review. Advances in Bioscience and Biotechnology 4:170-182.

Liu L, Ju JC and Yang X (1998). Differential inactivation of maturation promoting factor and mitogen-activated protein kinase following parthenogenetic activation in bovine oocytes. Biology of Reproduction 59:537-45.

Loi P, Ledda S, Fulka J, Cappai P and Moor RM (1998). Development of parthenogenetic and cloned ovine embryos: Effect of activation protocols. Biology of Reproduction 58:1177-87.

Nakada K and Mizuno J (1998). Intracellular calcium responses in bovine oocytes induced by spermatozoa and by reagents. Theriogenology 50:269-282.

Petr J, Chmelikova E, Dorflerova A, Jeseta M and Kuthanova Z (2007). Effects of protein kinase C on parthenogenetic activation of pig oocytes using calcium ionophore or nitric oxide-donor. Czech Jounral of Animal Science 52(12):415-422.

Puri Gopal and Bag Sadhan (2014). Generation of parthenogenetic embryos by ethanol activation in buffalo. Ruminant Science 3(1):5-7.

Sadeesh EM, Shah Fozia, Balhara AK, Thirumaran SMK, Balhara Sunesh and Yadav PS (2013). Effect of thiol compound supplemented culture medium on in vitro maturation of buffalo oocytes. Ruminant Science 2(1):59-62.

Sedmikova M, Burdova J, Petr J, Etrych M, Rozinek J and Jílek F (2003). Induction and activation of meiosis and subsequent parthenogenetic development of growing pig oocytes using calcium ionophore A23187. Theriogenology 60:1609-1620.

Shirazi A, Bahiraee A, Ahmadi E, Nazari H, Heidari B and Borjian S (2009). The Effect of the duration of in vitro maturation (IVM) on parthenogenetic development of ovine oocytes. American Journal of Molecular Biology 1:181-191.

Singh Vijay, Misra AK, Kumar Suresh and Kumar Vijay (2015a). Effect of seasons on in-vitro maturation and cleavage of oocyte and in-vitro development of buffalo embryo. Ruminant Science 4(1):29-31.

Singh Vijay, Misra AK, Kumar Suresh and Kumar Vijay (2015b). Effect of growth factors on in vitro maturation and in vitro culture of buffalo oocytes. Ruminant Science 4(2):219-224.

Swann K and Ozil JP (1994). Dynamics of the calcium signal that triggers mammalian egg activation. International Review of Cytology 1152:183-222.

Vrana KE, Hipp JD, Goss AM, McCool BA, Riddle DR, Walker SJ, Wettstein PJ, Studer LP, Tabar V, Cunniff K, Chapman K, Vilner L, West MD, Grant KA and Cibelli JB (2003). Nonhuman primate parthenogenetic stem cells. Proceedings of the National Academy of Sciences of the United States of America Vols 100:11911-11916.

Yang X, Presicce GA, Moraghan L, Jiang S and Foote RH (1994). Synergistic effect of ethanol and cycloheximide on activation of freshly matured bovine oocytes. Theriogenology 41:395-403