Assessment of in vitro maturation of oocytes derived from slaughterhouse goat ovaries
Title: Assessment of in vitro maturation of oocytes derived from slaughterhouse goat ovaries
Authors: Dharmendra Kumar, MK Shukla, LM Jeena, S Rahangdale, D Kumari, A Singh and BC Sarkhel
Source: Ruminant Science (2016)-5(1):17-20.
Cite this reference as: Kumar Dharmendra, Shukla MK, Jeena LM, Rahangdale S, Kumari D, Singh A and Sarkhel BC (2016). Assessment of in vitro maturation of oocytes derived from slaughterhouse goat ovaries. Ruminant Science 5(1):17-20.
The present study assessed the competency of different qualities of cumulus oocyte complexes (COC’s) for in vitro maturation (IVM) in goats. The COC’s were recovered from slaughterhouse ovaries and graded as A, B and C on the basis of cumulus mass. Out of total 2537 aspirated oocytes, 533 (21.01%), 1245 (49.07%) and 759 (29.92%) were graded as grade-A, B and C, respectively. On IVM, the excellent degree of cumulus expansion was found to be significantly higher (P<0.01) in grade-A (89.29±0.48%) as compared to grade-B oocytes (19.35±0.12%). The grade-B oocytes showed significantly higher rate of moderate (59.12±0.12 vs 9.04±0.63%) and poor (21.53±0.19 vs 4.66±0.74%) cumulus expansion as compared to A-grade oocytes. The nuclear maturation, indicated by first polar body formation in grade-A (95.25±0.82%) was significantly higher (P<0.01) than grade-B oocytes (90.68±0.50%). Our data clearly indicated that cumulus mass have significant roles and impacts on oocytes maturation.
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