Diagnosis and therapeutic management of hemorrhagic septicemia: a case report in 4 buffaloes

Diagnosis and therapeutic management of hemorrhagic septicemia: a case report in 4 buffaloes

Title: Diagnosis and therapeutic management of hemorrhagic septicemia: a case report in 4 buffaloes

Authors: Ravi Shankar Kumar Mandal, Aishwarya Lekshman, Ankush Rana, Chandrakiran Gaykwad, Surender Kumar, Abinav Suthar N, Prasanna Vadhana, DB Mondal and SK Dixit

Source: Ruminant Science (2016)-5(2):287-289.

Cite this reference as: Mandal Ravi Shankar Kumar, Lekshman Aishwarya, Rana Ankush, Gaykwad Chandrakiran, Kumar Surender, Suthar NA, Vadhana Prasanna, Mondal DB and Dixit SK (2016). Diagnosis and therapeutic management of hemorrhagic septicemia: a case report in 4 buffaloes. Ruminant Science 5(2):287-289.

Abstract

Four buffaloes with the history of pyrexia, brisket odema along with severe respiratory distress were presented to Referral Veterinary Polyclinic (RVP), IVRI. The cases were tentatively diagnosed as Hemorrhagic Septicemia and therapy was initiated against the same. For confirmatory diagnosis bacterial culture and Polymerase chain reaction (PCR) of the collected samples were performed. Leukocytosis with neutrophilia and mild reduction in hemoglobin level observed on hematological examination. Bacteriological culture of blood sample revealed non hemolytic small glistening mucoid dew-drop like colony on blood agar. On leishmann staining bipolar organism and on gram staining gram negative coccobacilli organism detected. Multiplex capsular PCR confirmed Pasteurella multocida type B. Antibiotic sensitivity test shows it is resistant to sulphamethaxazole+ Trimethoprime combination and sensitive to pencillins and cephalosporins On basis of the clinical signs and diagnostic plan the case was confirmed as Hemorrhagic Septicemia due to P. multocida type B and managed accordingly.

References

Annas S, Zamri-Saad M, Jesse FFA and Zunita Z (2014). New sites of localisation of Pasteurella multocida B: 2 in buffalo surviving experimental haemorrhagic septicaemia. BMC Veterinary Research 10(1):1.

Benkirane A and de Alwis MCL (2002). Haemorrhagic septicaemia, its significance, prevention and control in Asia. VeterinarnI Medicina (Praha) 47(8):234-240.

de Alwis MC and Sumanadasa  MA (1982). Naturally acquired immunity to haemorrhagic septicaemia among cattle and buffaloes in Sri Lanka. Tropical Animal Health and Production 14(1):27-28.

de Alwis MCL (1992). Haemorrhagic septicaemia-A general review. British Veterinary Journal 148(2): 99-112.

Horadagoda NU, Hodgson JC, Moon G M, Wijewardana TG and Eckersall PD (2002). Development of a clinical syndrome resembling haemorrhagic septicaemia in the buffalo following intravenous inoculation of Pasteurella multocida serotype B: 2 endotoxin and the role of tumour necrosis factor alpha. Research in Veterinary Science 72(3):194-200.

Ranjan R, Panda S, Acharya A, Singh A and Gupta M (2011). Molecular diagnosis of hemorrahagic septicemia-A review. Veterinary World 4(4):189-192.

Tabatabaei M, Jula GM, Jabbari AR and Esmailzadeh M (2007). Vaccine efficacy in cattle against hemorrhagic septicemia with live attenuated aroA mutant of Pasteurella multocida B: 2 strain. Journal of Cell and Animal Biology 1(4):62-65.