Fertility response to insulin modified Ovsynch protocol in postpartum acyclic buffaloes

Fertility response to insulin modified Ovsynch protocol in postpartum acyclic buffaloes

Title: Fertility response to insulin modified Ovsynch protocol in postpartum acyclic buffaloes

Authors: S Singh, SN Shukla and KK Gupta

Source: Ruminant Science (2019)-8(1):45-48.

Cite this reference as: Singh S, Shukla SN and Gupta KK (2019). Fertility response to insulin modified Ovsynch protocol in postpartum acyclic buffaloes. Ruminant Science 8(1):45-48.


The fertility response to various fixed time artificial insemination protocols was studied in terms of oestrus synchronization and conception rate on sixty postpartum buffaloes. The selected buffaloes were randomly divided into five groups, each comprising twelve (n=12) animals. Buffaloes of group I received GnRH and PGF2α as per Ovsynch protocol [GnRH at day 0 and 9 and PGF2α at day 7]; group II buffaloes received treatment similar to group I except half dose of GnRH at day 9; group III buffaloes received similar treatment as group I except additional administration of insulin (0.25 IU/kg. b.wt.) on day 8, 9 and 10; group IV animals were treated similar to group III except half dose of GnRH at day 9;  group V animals were received treatment similar to group I except replacement of second GnRH injection with insulin on day 8, 9 and 10. Fixed time insemination by frozen semen was done at 60 and 72 h after PGF2α administration in all the buffaloes and pregnancy was confirmed at 60 days post insemination. The results indicated satisfactory and comparable synchronization of oestrus (83.33 to 100%) and conception rate (41.66 to 66.66%) among the various treatment groups. The fertility response following insulin modified Ovsynch based timed AI protocols in postpartum buffaloes were found quite satisfactory and comparable.


Baruselli PS, Carvalho NAT, Gimenes LU and Crepaldi GA (2007). Fixed-time artificial insemination in buffalo. Italian Journal of Animal Science 6:107-118.                                                                                                                                                                 

Baruselli PS, Madureira EH, Barnabe VH, Barnabe RC, Visintin JA, Oliveira CA and Amaral R (1999). Estudo da dinamica follicular em bufalas submetidas a sincronizacao da ovulacao  para inseminacao artificial em tempo fixo. Arquivos da Faculdade de Veterinaria, UFRGS27: 210.

Derar R, Hussein HA, Fahmy S, El-Sherry TM and Megahed G (2012). Ovarian response and progesterone profile during the Ovsynch protocol in buffalo heifers and post-partum buffalo-cows (Bubalus bubalis). Buffalo Bulletin 31:52-60.

Dudi V, Mehta JS, Chaudhary AK, Kumar Pramod, Kumar Amit and Ruhil Swati (2017). Efficacy of different estrus synchronizing protocols on estrus induction in postpartum lactating dairy cows. Ruminant Science 6(2):337-340.

Fricke PM, Guenther JN and Wiltbank MC (1998). Efficacy of decreasing the dose of GnRH used in a protocol for synchronization of ovulation and timed AI in lactating dairy cows. Theriogenology 50:1275-1284. 

Gong JB, McBride D, Bramley TA and Webb R (1994). Effects of recombinant bovine somatotropin insulin-like growths factors-I and insulin on bovine granulosa cell steroidogenesis in vitro. Journal of Reproduction and Fertility110:611-622.

Gupta V, Thakur MS, Agrawal RG, Quadri MA and Shukla SN (2010). Effect of pre-treatment with insulin on ovarian and fertility response in true anestrus buffaloes to gonadotrophin-releasing hormone. Buffalo Bulletin 29(3):172-179.

Kumar Amit, Mehta JS, Ruhil Swati and Purohit GN (2018). Effects of different estrous synchronization protocols on estrus and subsequent fertility in cycling cows. Ruminant Science 7(1):83-86.

Kumar PR, Shukla SN, Shrivastava OP, Mishra, A and Purkayastha RD (2013). Therapeutic management of true anoestrus in dairy Buffaloes (Bubalus bubalis) using PMSG with metabolic Hormone. Veterinary Practitioner 14(2):323-324.

Kumar PR, Singh SK, Kharche SD, Govindaraju CS, Behera BK, Shukla SN, Kumar H and Agarwal SK (2014). Anoestrus in cattle and buffalo: Indian perspective. Advances in Animal and Veterinary Sciences 2(3):124-138.

Malik RK, Singh P, Singh IJ, Sharma RK, Phulia SK, Tuli RK and Chandolia RK (2011). Ovarian response and fertility of ovsynch-treated postpartum anestrus Murrah buffaloes. Buffalo Bulletin 30:4.

Palma GA, Muller M and Brem G (1997). Effect of insulin-like growth factor I (IGF-I) at high concentrations on blastocyst development of bovine embryos produced in vitro. Journal of Reproduction and Fertility 110:347-353.

Paul V and Prakash BS (2005). Efficacy of the Ovsynch protocol for synchronization of ovulation and fixed time artificial insemination in Murrah buffaloes (Bubalus bubalis). Theriogenology 64:49-106.

Purkayastha RD (2012). Efficacy of GnRH and insulin in true anestrus buffaloes under field conditions. MVSc-AH Thesis submitted to NDVSU, Jabalpur, MP.

Ramoun AA, Serur BH, Fattouh El SM, Darweish SA and Abou El Ghait HA (2012). Enhancing follicular growth as a prerequisite for GnRH treatment of true anestrum in buffalo. Animal Reproduction Science 13:29-35.

Renesis FD, Ronchi G, Guarneri P, Nguyen BX, Presicce GA, Huszenicza G and Scaramuzzi RJ (2005). Efficacy of the Ovsynch protocol for synchronization of ovulation and fixed time artificial insemination in Murrah buffaloes (Bubalus bubalis). Theriogenology 63(7):1824-1831. 

Sharma A, Shukla SN and Agrawal RG (2012). Administration of insulin and GnRH in repeat breeding cross bred cows. Indian Veterinary Journal 89(7):22-24.S

Shukla MK and Garg Abhishek (2012). Efficacy of different route of administration and doses of dinoprost in estrus induction/ synchronization in subestrus murrah buffaloes. Ruminant Science 1(1):63-65.  

Shukla SN, Agarwal SK, Shanker U, Varshney VP and Majumdar AC (2005). Modulation of ovarian response in anestrus cattle treated with Insulin alone and in combination with GnRH. Indian Journal of Animal Reproduction 26(2):159-164.

SimpsonRB, Chase CC, Spicer LJ, Vernon RK, Hammond AC and Rac DO (1994). Effect of exogenous insulin on plasma and follicular insulin like growth factor-I, insulin like growth factor binding protein activity, follicular estradiol and progesterone and follicular growth in super-ovulated Angus and Brahman cows. Journal of Reproduction and Fertility 102:483-92.

Singh AK, Shukla SP, Nema SP and Shukla MK (2014). Effect of different therapies on estrus expression of subestrus crossbred cows. Ruminant Science 3(2):245-246.

Singh Vijay, Misra AK, Kumar Suresh and Kumar Vijay (2015). Effect of growth factors on in vitro maturation and in vitro culture of buffalo oocytes. Ruminant Science 4(2):219-224.

Stewart RE, Spicer LJ, Hamilton TD and Keefer BE (1995). Effects of insulin like growth factor-I and insulin on proliferation and on basal luteinizing hormone induced steroidogenesis of bovine thecal cells: Involvement of glucose and receptors for insulin-like growth factor-I and luteinizing hormone. Journal of Animal Science 73:3719-3731.

Tanaka T, Nagatani S, Bucholtz DC, Ohkura S, Tsukamura H, Maeda K and Foster DL (2000). Central action of insulin regulates pulsatile luteinizing hormone secretion in the diabetic sheep model. Biology of Reproduction 62(5):1256-1261.

Yamada K, Nakao T, Nakada K and Matsuda G (2002). Influence of GnRH analogue (fertirelin acetate) doses on synchronization of ovulation and fixed-time artificial insemination in lactating dairy cows. Animal Reproduction Science 74:27-34.