Identification of candidate K232A polymorphism of DGAT1 gene in Hariana cattle
Title: Identification of candidate K232A polymorphism of DGAT1 gene in Hariana cattle
Authors: Rajpal Kaur, Ankit Magotra, Ramkaran, Sandeep Gera, Sandeep Kumar, Kamaldeep, Asha Garg and Rahul
Source: Ruminant Science (2018)-7(1):33-36.
Cite this reference as: Kaur Rajpal, Magotra Ankit, Ramkaran, Gera Sandeep, Kumar Sandeep, Kamaldeep, Garg Asha and Rahul (2018). Identification of candidate K232A polymorphism of DGAT1 gene in Hariana cattle. Ruminant Science 7(1):33-36.
DGAT1 gene is considered to be a very strong positional candidate gene for fat per cent of milk. The present study was conducted on 50 Hariana cattle to screen candidate lysine to alanine substitution (K232A) polymorphism significantly associated with milk production traits. The PCR product of 413 bp was digested with EaeI restriction enzyme to screen the reported mutation. All the animals were of KK genotype and found to be monomorphic. The result indicates highly conserved sequence in Hariana cattle under study.
Ashwell MS, Van Tassell CP and Sonstegard (2001). A genome scan to identify quantitative trait loci affecting economically important traits in a US Holstein population. Journal of Dairy Science 84(11):2535-2542.
Bovenhuis H and Schrooten C (2002). Quantitative trait loci for milk production traits in dairy cattle. In: Proceedings of the 7th World Congress on Genetics Applied to Livestock Production, Montpellier, France.
Cases S, Smith SJ, Zheng YW, Myers HM, Lear SR, Sande E, Novak S, Collins C, Welch CB, Lusis AJ and Erickson SK (1998). Identification of a gene encoding an acyl CoA: diacylglycerolacyl transferase, a key enzyme in triacylglycerol synthesis. Proceedings of the National Academy of Sciences of the United States of America 95(22):13018-13023
Ganguly I, Kumar S, Gaur GK, Singh U, Kumar A, Kumar S and Mann S ( 2013). DGAT1 polymorphism K232A in Sahiwal (Indian Zebu) and Frieswal (Holstein friesian X Sahiwal crossbred) cattle. Indian Journal of Animal Research 47(4):360-363.
Grisart B, Coppieters W, Farnir F, Karim L, Ford C, Berzi P, Cambisano N, Mni M, Reid S, Simon P and Spelman R (2002). Positional candidate cloning of a QTL in dairy cattle: Identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition. Genome Research 12(2):222-231.
Heydarian D, Miraei-Ashtiani SR and Sadeghi M (2014). Study on DGAT1-exon8 polymorphism in Iranian buffalo. International Journal of Advanced Biological and Biomedical Research 2(7):2276-2282.
Heyen DW, Weller JI, Ron M, Band M, Beever JE, Feldmesser E, Da Y, Wiggans GR, VanRaden PM and Lewin HA (1999). A genome scan for QTL influencing milk production and health traits in dairy cattle. Physiological Genomics 1(3):165-175.
Kaupe B, Winter A, Fries R and Erhardt G (2004). DGAT1 polymorphism in Bos indicus and Bos taurus cattle breeds. Journal of Animal Science 71(02):182-187.
Komisarek J, Waskowicz K, Michalak A and Dorynek Z (2004). Effects of DGAT1 variants on milk production traits in Jersey cattle. Animal Science Papers and Reports 22(3):307-313.
Lacorte GA, Machado MA, Martinez ML, Campos AL, Maciel RP, Verneque RS, Teodoro RL, Peixoto MGCD, Carvalho MRS and Fonseca CG (2006). DGAT1 K323A polymorphism in Brazilian cattle breeds. Genetics and Molecular Research 5:475-482.
Pareek CS, Czarnik U, Zabolewicz T, Pareek RS and Walawski K (2005). DGAT1 K232A quantitative trait nucleotide polymorphism in Polish Black-and-White cattle. Journal of Applied Genetics 46(1):85-87.
Rahmatalla S, Mueller U, Strucken EM and Brockmann GA (2008). Characterization of DGAT1-variants in German Holstein cattle under production conditions. Zuchtungskunde 80(60):473-484.
Sambrook J and Russell DW (2001). Molecular Cloning: A Laboratory Manual. Cold Spring laboratory Press, New York.
Sara Abu SM, Said Ahmed A, Ahmed MKA, Reissmann M, Brockmann GA and Rahmatalla SA (2015). Variants of the diacylglycerolacyl transferase 1 (DGATl) gene in Sudanese dairy cattle (Kenana and Butana). Journal of Molecular Genetics 7(1-4):5-9.
Schennink A, Stoop W, Visker M, Heck J, Bovenhuis H,Van Der PoelJJ, Van Valenberg HJ and Van Arendonk JA (2007). DGAT1 underlies large genetic variation in milk-fat composition of dairy cows. Animal Genetics 38:467-473.
Spelman RJ, Ford CA, McElhinney P, Gregory GC and Snell RG (2002). Characterization of the DGAT1 gene in the New Zealand dairy population. Journal of Dairy Science 85:3514-3517.
Tantia MS, Vijh RK, Mishra BP, Mishra B, Kumar SB and Sodhi M (2006). DGAT1 and ABCG2 polymorphism in Indian cattle (Bos indicus) and buffalo (Bubalus bubalis) breeds. BMC Veterinary Research 2(1):32.
Thaller G, Kramer W, Winter A, Kaupe B, Erhardt G and Fries R (2003). Effects of variants on milk production traits in German cattle breeds. Journal of Animal Science 81(8):1911-1918.
Venkatachalapathy RT, Sharma A and Radha K (2014). Polymorphism at DGAT1 locus in Indian buffalo, zebu and Bos indicus × Bos taurus cattle breeds. Veterinary Science Research Journal 5(1/2):13-17.
Winter A, Kramer W, Werner FA, Kollers S, Kata S, Durstewitz G, Buitkamp J, Womack JE, Thaller G and Fries R (2002). Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl-CoA: diacylglycerolacyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content. Proceedings of the National Academy of Sciences of the United States of America 99(14):9300-9305.